Fluorescent Polystyrene Microspheres


Fluorescent polystyrene microspheres are monodisperse microspheres containing high-brightness fluorescent dyes. The fluorescent dyes are embedded in the microspheres, which can effectively prevent fluorescence quenching and reduce the influence of environmental factors on the fluorescence intensity. Some functional groups have no effect on immunoassays, detection, etc. This range of particle emission wavelengths covers the entire visible light range from 460 nm to 750 nm (excitation wavelengths are typically 30 – 40 nm lower than emission wavelengths).


Perfect spherical, highly uniform particle size(CV<3%)

High chemical stability

Good monodispersity


Fluorescent polystyrene microspheres can be widely used in lateral chromatography, phagocytosis studies, pore size determination, biochips, microfluidics and other scientific research fields.


Store at 2 – 8°C, do not freeze.

Technical Index
Material Polystyrene


Particle diameter 0.05-20μm
Solid content 1% w/v
Surface modified with modified carboxyl groups
Preserving fluid Pure water(containing 0.05%w/v Proclin®300)



Maximum excitation wavelength Maximum excitation wavelength
Blue fluorescence FB 430nm 460nm
Green fluorescence FG 495nm 515nm
Yellow fluorescence FY 540nm 560nm
Orange fluorescence FO 560nm 605nm
Red fluorescence FR 625nm 640nm
Dark red fluorescence FD 660nm 680nm
Time-resolved fluorescence FT 365nm 610nm


Fluorescent polystyrene microspheres with modified carboxyl groups, Time-resolved fluorescence
0.10μm 0.20μm 0.30μm
Time-resolved fluorescence(365/610nm) LFsphere®010PS LFsphere®020PS LFsphere®030PS
Fluorescent polystyrene microspheres with modified carboxyl groups, green fluorescence
0.05μm 0.10μm 0.20μm 0.50μm 1.0μm
Green fluorescence(495/515nm) LFsphere®005PS LFsphere®010PS LFsphere®020PS LFsphere®050PS LFsphere®100PS
2.0μm 5.0μm 10μm 20μm
LFsphere®200PS LFsphere®500PS LFsphere®1000PS LFsphere®2000PS
Fluorescent polystyrene microspheres with modified carboxyl groups, other fluorescence
Yellow fluorescence 0.12μm 0.3μm
Dark red fluorescence LFsphere®012PS

Packaging sizes:1/10/50/100ml